FIG. 3.

Northern blot (A and C) and PhosphorImager (B and D) analysis showing the effects of increasing expression of NS1 plasmid on the synthesis of positive-sense RNA from the C2 minigenome (A and B) and the synthesis of negative-sense RNA from the C4 mini-antigenome (C and D). 293 cells were infected with vaccinia virus vTF7-3 and transfected with 50 ng of C2 minigenome (A) or C4 mini-antigenome (C) plasmid, 200 ng of N plasmid, 100 ng of P plasmid, 0 ng (lane 1) or 25 ng (lanes 2 to 10) of L plasmid, 0 ng (top panel) or 10 ng (bottom panel) of M2 plasmid containing both ORFs, and the indicated amount (0 to 30 ng [lanes 2 to 10]) of NS1 plasmid. Intracellular RNA was harvested 40 h postinfection and analyzed by Northern blot hybridization with a negative-sense (A) or positive-sense (C) RSV-CAT riboprobe. Hybridized radioactivity was quantitated by PhosphorImager analysis: quantitation of panels A and C is shown in panels B and D, respectively.