TABLE 2.
Induction of SV40-specific CTL by immunization of C57BL/6 mice with vaccinia virus recombinants expressing epitope V minigene derivatives
| Expt and rVV used for immunizationa | % Specific target cell lysisb
|
|||
|---|---|---|---|---|
| RMA + LT206-215 | RMA + LT489-497 | RMA + DbN5 | B6/T117A1 | |
| 1 | ||||
| rVV-V (cl4) | 3 | 3 | 3 | 2 |
| rVV-ES-V | 6 | 76 | 5 | 42 |
| rVV-A2-V | 4 | 35 | 5 | 18 |
| rVV-A2-V-A2 | 4 | 13 | 5 | 5 |
| rVV-V-A2 | 4 | 46 | 3 | 17 |
| rVV-I-V-II/III | 87 | 54 | 7 | 42 |
| rVV-I-Db-II/III | 82 | 4 | 3 | 2 |
| 2 | ||||
| rVV-V (cl2) | 3 | 2 | 2 | NDc |
| rVV-ES-V | 8 | 50 | 4 | ND |
| rVV-A2-V | 8 | 47 | 6 | ND |
| rVV-A2-V-A2 | 11 | 47 | 6 | ND |
| rVV-V-A2 | 7 | 29 | 3 | ND |
| rVV-I-V-II/III | 100 | 44 | 9 | ND |
| rVV-I-Db-II/III | 100 | 14 | 13 | ND |
a
Groups of two or four C57BL/6 mice were immunized i.v. with 107 PFU of vaccinia virus recombinants. Splenocytes were restimulated in vitro with B6/WT-19 cells for 6 days and used in a 51Cr release assay at an E/T ratio of 50:1.
b
51Cr-labeled RMA cells were incubated in RPMI medium containing peptide at a final concentration of 1 μM. B6/T117A1 cells express a derivative of Tag lacking epitopes I, II/III, and IV and were induced with gamma interferon prior to the assay. LT206-215, epitope I; LT489-497, epitope V; DbN5, SMIKNLEYM.
c
ND, not done.