TABLE 3.
Induction of epitope V-specific CTL by murine DHFR derivatives bearing an epitope V insert flanked at the amino terminus by an alanine or methionine residue
| rVV used for immunizationa | % Specific target cell lysis
|
|||
|---|---|---|---|---|
| RMA cells pulsed withb:
|
SV40 Tag-expressing cells
|
|||
| LT489-497 | DbN5 | B6/T117A1 | B6/K-1,4,5 | |
| rVV-ES-V | 62 | 3 | 44 | 3 |
| rVV-Dhfr/A-V | ||||
| cl1 | 20 | 9 | 20 | 5 |
| cl2 | 46 | 13 | 24 | 8 |
| rVV-Dhfr/M-V | ||||
| cl1 | 44 | 12 | 36 | 4 |
| cl2 | 29 | 5 | 24 | 3 |
| rVV-Dhfr/ | 9 | 9 | 10 | 5 |
a
Groups of four C57BL/6 mice were immunized i.v. with 107 PFU of vaccinia virus recombinant. Splenocytes were restimulated in vitro for 6 days with γ-irradiated B6/WT-19 cells and used as effectors in a 51Cr release assay at a 50:1 E/T ratio.
b
51Cr-labeled RMA cells were incubated in the presence of 1 μM peptide in RPMI medium. LT489-497, epitope V, QGINNLDNL; DbN5, SMIKNLEYM.