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Sedimentation and BS3-cross-linking profiles of gp41 mutants containing two ectodomain lysines. Lysates from HeLa cells expressing the indicated Lys-to-Arg Env mutants were treated as described in the legend to Fig. 2. Env glycoproteins were visualized by immunoblotting with human α-588-599 antibody and 125I-protein A. The direction of sedimentation is right to left and is indicated by an arrow. ∗, unfractionated cell lysates; FRAC, fraction.
