Table 1.
Summary table listing all publications discussed in this manuscript on ex vivo lung perfusion.
| Study | Animal species/strain | Perfusion solution | Tubing type/length | Use of oxygenators | Flow rate | Length of perfusion | Temperature (°C) | Application | Functional tests performed | Cannulation site |
|---|---|---|---|---|---|---|---|---|---|---|
| Lung | ||||||||||
| Benchtop | ||||||||||
| Maxey, 2004 | C57BL/6 mice (adult males) | Krebs–Henseleit buffer containing 2% albumin, 0.1% glucose, and 0.3% HEPES (335–340mOsm/kg) | Not described | No | 2 mL/min | 60 min | 37°C | Modeling pulmonary I/R injury | Lung weight, lung injury score, pulmonary artery and vein pressures, airway resistance, pulmonary compliance | Trachea for ventilation; inferior vena cava for media perfusion |
| Zhao, 2006 | C57BL/6 mice (adult males) | Krebs–Henseleit buffer containing 2% albumin, 0.1% glucose, and 0.3% HEPES (335–340mOsm/kg) | Not described | No | 60 μL/g body weight/min | 60 min | 37°C | Investigating the role of alveolar macrophages in pulmonary I/R injury | Lung weight, cytokine/chemokine expression (RT-PCR, ELISA), lung vascular permeability, neutrophil quantification, pulmonary artery pressure, pulmonary compliance | Trachea for ventilation; inferior vena cava for media perfusion |
| Francioli, 2017 | Sprague–Dawley rat (300 g) | Steen® solution (Xvivo perfusion, Göteborg, Sweden, pH 7.4) | Not described | Gas-exchange membrane | Not described | 4 h | 37°C | Testing of pharmaceutical drug pyrrolidine dithiocarbamate (PDTC) during warm ischemia | Physiological variables (PA pressure, arterial pressure, vascular resistance, compliance), protein concentration and lactate dehydrogenase activity in bronchoalveolar lavage, pro-inflammatory cytokine levels, NF-kB expression | Pulmonary artery for media perfusion; trachea for ventilation |
| Wang, 2016 | Sprague–Dawley rat (400 g) | Steen solution (Xvivo perfusion, Göteborg, Sweden, pH 7.4) | Not described | Gas-exchange membrane | Not described | 3 h | 37°C | Effect of MnTBAP and 3-AB on the lung during warm ischemic injury | Physiological variables (PA pressure, arterial pressure, vascular resistance, compliance), protein concentration and lactate dehydrogenase activity in bronchoalveolar lavage, pro-inflammatory cytokine levels, histology | Pulmonary artery for media perfusion; trachea for ventilation |
| Incubator | ||||||||||
| Mishra, 2012 | Sprague–Dawley rats (6-12-week-old male) | RPMI | Silicone (3 m) | No | 6 mL/min | 7–14 days | 37°C | Modeling human lung cancer on a decellularized rat lung matrix | Histology, DNA extraction, immunohistochemistry | Trachea for cell seeding, pulmonary artery for media perfusion |
| Ahmadipour, 2021 | C57BL/6 mice (12-14-week-old males) | High glucose content DMEM containing 10% FBS and 1% antibiotics | Not described | No | 1.5 mL/min (vascular perfusion rate); 40 breaths/min with a tidal volume of 300 μL (ventilation rate) | 3 days | 37°C | Describing a novel ventilation protocol for enhanced recellularization of rat lung scaffolds | Histology, immunofluorescence staining, immunohistochemistry, RT-PCR | Trachea for liquid ventilation and cell seeding, pulmonary artery for media perfusion |
| Engler, 2018 | Sprague–Dawley rat (200 g) | DMEM high glucose with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, 3 μg/mL amphotericin B and 50 μg/mL gentamicin | Silicone (1.5 m) | Hollow fiber cartridge oxygenator | 4 mL/min (perfusion rate); 4, 6, 8, 16, or 32 mL/min (oxygenator flow rate) | 24 h | 37°C | Mathematical modeling and characterization of oxygen requirements of whole lung tissues | Histology, immunofluorescence staining, whole lung cell count, total lung weight, single cell oxygen consumption rate, glucose consumption, lactate production | Pulmonary artery for media perfusion |