Table 4.
Summary table listing all publications discussed in this manuscript on ex vivo pancreas perfusion.
| Study | Animal species/strain | Perfusion solution | Tubing type/length | Use of oxygenators | Flow rate | Length of perfusion | Temperature (°C) | Application | Functional tests performed | Cannulation site |
|---|---|---|---|---|---|---|---|---|---|---|
| Pancreas | ||||||||||
| Benchtop/incubator | ||||||||||
| Single-pass method (Pegg, 1982) | WAG rats | Gelatin polypeptide Haemaccel + 5.4 mM calcium + glucose (5 mM for first hour, 30 mM for second hour) | Not described | Bubbled oxygen (95% O2, 5% CO2) | 2 mL/min | 2 h | 38°C | To test assays for measuring glucose-responsiveness | Insulin secretion assay (5 mM vs. 30 mM glucose) | Input: Aorta (below superior mesenteric artery) |
| Recirculation method (Pegg, 1982) | WAG rats | Gelatin polypeptide Haemaccel + 5.4 mM calcium + 5 mM glucose | Not described | Bubbled oxygen (95% O2, 5% CO2) | 60 mmHg (pressure) | 2 h | 38°C | To test assays for measuring glucose-responsiveness | Insulin secretion assay (5 mM vs. 30 mM glucose) | Input: Aorta (below superior mesenteric artery) |
| Loubatieres-Mariani, 1980 | Wistar rats | Krebs–Ringer bicarbonate buffer + 2 g/L purified bovine albumin + 0–3 g/L glucose | Not described | Bubbled oxygen (93% O2, 7% CO2) | 2.4 mL/min | 90 min | 28°C & 37.5°C | To test effect of temperature (hypothermia) on glucose responsiveness | Insulin secretion assay (following stimulation with high glucose, tolbutamide, acetylcholine, and glucagon) | N/A |
| Kojima, 1984 | Wistar rats | Na+; 131 mEq/1, lactate-: 28 mEq/L, K+: 4 mEq/1, Ca∗: 3 mEq/L, CI-: 110 mEq/1, Dextran 40:30.0 gr/1, glucose: 1 gr/1, pH: 8.2 + 0.2. T | Not described | Artificial lung | 10 mL/min | 6 h | 4°C (hypothermic) | To test the effect of hypothermia, hyperbaria, and oxygenation during pancreas preservation on islet health and function | Insulin secretion assay; transplantation into diabetic mice for reversal of diabetes | Input: celiac axis |
| Klempanauer, 1982 | Wistar rats | Protide gel solution, fructose-bicarbonate solution, modified cryoprecipitated plasma, Collins' C3 solution, Sack's solution | Not described | No | Not described | 24 h | 0°C–4°C | To demonstrate that a simpler approach, using initial perfusion with a chilled solution, following by storage at 0–4C is superior to other methods for 24 h perfusion | N/A | Input: Aorta (below superior mesenteric artery) |
| Mantke, 2009 | Wistar rats | Modified Krebs–Ringer bicarbonate | Not described | 95% O2, 5% CO2 | 1 mL/min (220 mmHg) | 80 min (60 min perfusion time, 20 min equilibration time) | 37°C (using heat exchanger) | To examine early mechanisms leading to acinar cell injury in acute pancreatitis | Amylase, lipase, and lactate dehydrogenase activity | Causal aorta (input), portal vein (output). Both cannulated with Teflon tube |