Table 1.

Requirements for the installation of a BSL-3 laboratory.

Aspect	Requirements
Physical structure	The structure must have sufficient space for the allocation of equipment and the safe development of activities
	The primary access must have a structure compatible with a BSL-2 laboratory
	Double-door antechamber that defines access flow and must be self-closing
	Controlled, restricted, and secure access to the BSL-3 area
	Exclusive technical floor
	Exclusive engineering facilities and systems
	Dedicated and dedicated emergency power system
	Voice and data communication system
Air-conditioning and exhaust system	Specific, exclusive, and independent air-conditioning system for the BSL-3 area
	The air-conditioning system must ensure the parameters of the differential pressure gradients and airflow
	The airflow in the facility should always be in a single direction, that is, from the least contaminated area to the most contaminated area
	Alarm for airflow monitoring to alert laboratory and engineering staff of any control set parameters
	Extract air may not be recirculated within the room or to any other area of the building
	Filtration through HEPAa filters
	Inflation and exhaust systems should be designed to maintain operational balance and perfect compatibility with BSC
Equipment and internal arrangements	Class II BSC is available inside the BSL-3 area
	Barrier autoclave provided inside the laboratory
	Specific washing/expurgation area
	Effluent treatment system
	Emergency showers and eye wash were provided at the exit sluice
	Sink, if existent, with hands-free tap
	Windows should be fixed and sealed
	Penetrations and finishes in the BSL-3 area should be sealed
	Luminaires, diffusers, and roof grilles should have their joints sealed to control air leakage from the containment environment
	Doors should allow for tightness during biological decontamination processes
	Benches and chairs should be waterproof and resistant to disinfectants, acids, alkalis, organic solvents, and moderate heat
	Furniture and equipment must allow for decontamination
	The finished floor should be impervious to liquids, with sealed seams, resistant to chemicals, and have a surface that reduces the danger of slipping

^aHEPA (high-efficiency particulate air): technology used in air filters with high efficiency in particle separation. In controlled environments, it is important for keeping the air free from contamination and preventing the spread of pathogens.