Table 3.
Cytotoxic activity of selected compounds in 2D and 3D cultures of the HCT116 colorectal cancer cell line, determined by the fluorimetric resazurin assay after 96 h of exposure.
| Compound | IC50 2D (μM) | IC50 3D (μM) | 3D/2D |
|---|---|---|---|
| 1a | 18 ± 5 | 31 ± 5 | 1.7 |
| 1c | 6.5 ± 1.3 | 8.9 ± 2.1 | 1.4 |
| 3a | 8.4 ± 0.7 | 10.6 ± 0.9 | 1.3 |
| 3c | > 200 | 226 ± 36 | < 1.1 |
| 4a | 9.6 ± 1.2 | 9.4 ± 4.6 | 0.98 |
| 4c | > 200 | 344 ± 19 | < 1.7 |
Note: IC50 values (means ± standard deviations) were determined from interpolated concentration–effect curves of at least three independent experiments.