TABLE 5.
Summary of findings for p15E mutants
Envelope protein | SU/TM processinga | Incorporationb | Cell-cell fusionc | Titerc | Phenotype |
---|---|---|---|---|---|
L441F | + | + | +++ | +++ | WTd |
L443R | − | − | − | − | Processing/incorporation defective |
L443A | − | Low | − | − | Processing/incorporation defective |
L443I | − | − | − | + | Processing/incorporation defective |
L445E | + | + | − | − | Fusion defective |
G449L | − | − | − | − | Processing/incorporation defective |
G449W | − | − | − | − | Processing/incorporation defective |
G449A | − | − | − | − | Processing/incorporation defective |
G449S | − | − | − | − | Processing/incorporation defective |
A457F | + | Low | − | − | Fusion defective |
G460V | + | + | ++ | + | Nearly WT |
G460E | + | + | − | − | Fusion defective |
T461P | + | + | − | − | Fusion defective |
T463A | + | + | +++ | + | Nearly WT |
T464A | + | + | ++++ | +++ | Hyperfusogenic, but WT titer |
T464I | + | + | +++ | ++ | Nearly WT |
T464K | + | + | +++ | ++ | Nearly WT |
M467E | + | + | − | + | No syncytia, but some titer |
A468R | + | + | +++ | +++ | WT |
Q474E | + | + | ++ | + | Nearly WT |
L475R | − | − | − | − | Processing/incorporation defective |
+, SU and TM proteins are processed; −, only Pr85 is present.
+, substantial incorporation; −, no incorporation; Low, low level of incorporation.
++++, value greater than the wild-type value; +++, 50 to 100% of wild-type value; ++, 10 to 50% of wild-type value; +, 1 to 10% of the wild-type value.
WT, wild type.