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Detection of the VP1 gene in transgenic plants by PCR. Plant DNA was isolated from cell extracts, and PCR was performed with a pair of primers designed to amplify the region between positions 369 and 490 of the VP1 gene. Lanes: A, nontemplate; B and E, DNA from pRok-transformed plants; C, D, and F, DNA from pRok.VP1-transformed plants; G, pRok.VP1 plasmid.
