FIG. 2.

Activity of NEM-alkylated M-MuLV IN proteins in strand transfer and unimolecular disintegration reactions. (MOCK) Lanes 1 to 8, odd and even numbered lanes are reactions that respectively contained 10 and 20 pmol of the mock-treated proteins; lanes 9 to 11, complementation reactions which contained 10 pmol each of the indicated mock-treated proteins. (NEM) Assays were performed with 10 pmol of each of the indicated proteins. M-MuLV IN proteins that were subjected to NEM alkylation are indicated by bold underlined characters. Non-underlined proteins are untreated IN. Lane 1, NEM-alkylated CΔ232 negative-control reaction; lanes 2 to 13, reactions that contained individual or mixed pairs of treated and untreated proteins. (A) Strand transfer reactions with substrate 2784/2785 (13, 24). (B) Unimolecular disintegration reactions with the standard Y substrate (Y3154ds [13, 14]). The position of the 5′ ³²P-labeled C strand of oligonucleotide 3152 (14) is indicated (•).