FIG. 5.

In vivo analysis of the effects of the C209A IN mutation on viral replication. (A) Time course of viral RT release into medium by Rat-1 producer cells. Days posttransfection are indicated on the left. Passages of the transfected producer cell line are indicated by arrows. (B) Accumulation of C209A viral RT in medium of plates maintained from passage on day 21 through day 40. (C) Time course of viral spread in Rat-1 cells. WT and C209A (pNCA-c/C209A IN) viruses collected at 40 days posttransfection were normalized for RT activity and used to infect fresh Rat-1 cells. RT activity was quantitated on a Molecular Dynamics PhosphorImager (Sunnyvale, Calif.). The pixel densities for each time point during the infection represent relative RT activity and are indicated on the y axis. The drop in RT activity at day 4 was due to passage of the infected cell lines. d.p.i., days postinfection.