Table 3.
Proteases and protease inhibitors identified in I. ricinus Midgut contents
| Protein annotation | Protein ID | Accession no. (major protein IDs) | National Center for Biotechnology Information accession no. | Razor + unique peptides | Unique + razor sequence coverage (%) | Mean transformed LFQ values (±SD) |
Tissue | |||
|---|---|---|---|---|---|---|---|---|---|---|
| FF | 4AD | 6AD | 11AD | |||||||
| Proteases | ||||||||||
| Cathepsin L1 (IrCL1) | 59 | A0A0K8REA0 | ABO26562 | 9 | 39.4 | 18.08 (0.20) | 17.71 (0.87) | Yes | ||
| Cathepsin long (Ir26/29) | 371 | V5GJA7 | JAB70370 | 4 | 19 | 16.61 (0.05) | 16.71 (0.18) | Yes | ||
| Cathepsin B1 isoform 3 (IrCB1.3) | 400 | V5HDT5 | JAB75624 | 7 | 37.4 | 17.88 (0.39) | 18.07 (0.73) | 17.71 (0.80) | Yes | |
| Cathepsin C (IrCC) | 266 | A8D0K2 | ABV29335 | 6 | 21.7 | 16.59 (0.39) | 16.62 (0.85) | 16.71 (0.34) | Yes | |
| Chymotrypsin-like nonpeptidase homolog (HD rich) | 127 | A0A131XYL3 | JAP70806 | 3 | 13 | 18.15 (0.27) | 18.60 (0.43) | 19.37 (0.78) | Yes | |
| Chymotrypsin-like nonpeptidase homolo (HD rich) | 396 | V5HAY7 | JAB70298 | 19 | 61.1 | 17.97 (0.73) | 20.29 (1.12) | 19.12 (0.54) | 20.75 (0.28) | Yes |
| Chymotrypsin-like nonpeptidase homolog (HD rich) | 402 | V5HFD4 | JAB72178 | 5 | 25.6 | 19.56 (1.23) | 17.60 (1.53) | 19.20 (0.58) | Yes | |
| Cubilin-related serine protease 2 | 257 | A0A6B0VD03 | JAB71130 | 3 | 9.5 | 16.36 (0.13) | 17.01 (0.22) | 17.17 (0.71) | Yes | |
| Trypsin-like serine protease | 388 | V5H4M9 | 3 | 17.5 | 16.66 (0.85) | No | ||||
| Protease inhibitors | ||||||||||
| Stefin | 150 | A0A131Y5V6 | JAP73872 | 2 | 33.3 | 14.99 (0.12) | Yes | |||
| Iristatin | 252 | A0A3B6UEB6 | ARQ18757 | 5 | 43.3 | 18.02 (0.45) | 18.26 (0.53) | 17.22 (0.54) | Yes | |
| Cystatin 1 | 360 | A0A131YB86 | JAP75211 | 6 | 69.9 | 18.77 (2.07) | 19.78 (0.66) | 19.26 (0.63) | Yes | |
| Cystatin 2 | 165 | A0A131Y7Z0 | JAP74937 | 9 | 57.1 | 18.29 (0.95) | 17.89 (1.67) | Yes | ||
| TIL-domain inhibitor | 97 | A0A0K8RNV1 | 4 | 53.3 | 18.36 (0.49) | 19.07 (0.47) | 20.15 (0.55) | 16.97 (0.52) | Yes | |
| TIL-domain inhibitor | 108 | A0A131XMQ1 | JAP68754 | 6 | 43.3 | 17.80 (1.61) | 19.65 (0.90) | 18.52 (0.68) | 16.94 (2.00) | Yes |
| TIL-domain inhibitor | 156 | A0A131Y6Y7 | 9 | 57.4 | 16.28 (1.34) | 19.48 (0.74) | 17.39 (1.65) | 14.50 (1.62) | Yes | |
| TIL-domain inhibitor | 389 | A0A131Y789 | JAP74697 | 5 | 49.5 | 18.77 (1.67) | 18.68 (1.25) | 19.45 (0.39) | Yes | |
| TIL-domain inhibitor | 414 | V5IDG7 | JAB72501 | 8 | 66.4 | 19.16 (1.16) | 21.17 (1.04) | 20.88 (0.40) | Yes | |
| TIL-domain inhibitor | 29 | A0A131Y8L3 | JAP75157 | 4 | 48.4 | 16.02 (0.50) | 17.11 (2.76) | 16.76 (0.29) | Yes | |
| TIL-domain inhibitor | 154 | A0A131Y6K8 | JAP74447 | 4 | 75 | 19.52 (0.82) | 21.17 (0.96) | 19.04 (1.96) | Yes | |
| TIL-domain inhibitor | 427 | W8JJG5 | 1 | 15.3 | 18.00 (0.71) | 17.47 (0.96) | No | |||
| Single Kunitz–domain inhibitor | 54 | V5H5D1 | JAB78230 | 3 | 39.4 | 19.91 (0.39) | 20.25 (0.12) | 22.30 (0.92) | 22.53 (0.72) | Yes |
| Multi Kunitz–domain (4×) inhibitor | 147 | A0A131Y5D8 | JAP73590 | 3 | 27.1 | 16.39 (1.06) | Yes | |||