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. 1998 Jan;64(1):43–52. doi: 10.1128/aem.64.1.43-52.1998

FIG. 1.

FIG. 1

Microtiter plate assay comparing the activity of purified ArfI (1st row) to activities present in a crude Triton extract of cells (3rd row) with the following 4-methylumbelliferyl derivatives: column 1, β-d-galactoside; column 2, α-d-galactoside; column 3, α-l-arabinopyranoside; column 4, α-l-arabinofuranoside; column 5, α-d-glucoside; column 6, β-d-glucoside; column 7, β-d-glucuronide; column 8, β-d-cellobioside; column 9, acetate; column 10, β-d-xyloside. Each well containing purified ArfI contained 48 ng of protein (equivalent to 10.6 U of ARAF activity). Each well containing Triton extract contained 4.2 μg of protein (equivalent to 0.011 U of ARAF activity). The control (2nd row) contained test substrate, but lacked enzyme. Incubation was for 12 h at RT.