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IEF gel of purified ArfI (1 μg of protein per lane). (A) Gel stained with crocein scarlet. (B) Gel overlaid with an MU-AF zymogram gel. Lane 1, purified ArfI; lane 2, purified ArfI plus pI standards (β-lactoglobulin B [pI 5.1], bovine carbonic anhydrase [pI 6.0], and human carbonic anhydrase [pI 6.5]).
