TABLE 1.
Summary of purification of ArfI from C. xylanolytica
| Purification stepa
|
Total amt of protein (mg) | Total activity (U)b | Sp act (U · mg−1) | Purifi- cation (fold) | Yield (%) | |
|---|---|---|---|---|---|---|
| No. | Column | |||||
| None (Triton extract) | 429.9 | 1,118 | 2.6 | 1.0 | 100.0 | |
| 1 | DEAE-cellulose | 183.9 | 1,067 | 5.8 | 2.2 | 95.4 |
| 2 | DEAE-Sephadex | 73.1 | 951 | 13.0 | 5.0 | 85.1 |
| 3 | Hydroxylapatite | 7.7 | 808 | 104.9 | 40.3 | 72.3 |
| 4 | Phenyl-Sepharose | 1.7 | 347 | 204.1 | 78.5 | 31.0 |
| 5 | Macro Prep 50Q | 1.6 | 291 | 181.9 | 70.0 | 26.0 |
| 6 | Phenyl-Sepharose | 1.2 | 264 | 220.0 | 84.6 | 23.6 |
a
See Materials and Methods for details.
b
Measured with pNP-AF as the substrate.