TABLE 3.
Distribution of ArfI and ENDOX activities in cells of C. xylanolytica
| Treatment | Cellular fraction | % of total activity or proteina
|
|||
|---|---|---|---|---|---|
| ArfI | ENDOX | GPD | Protein | ||
| Sonication | Soluble fraction | 96.6 | 60.3 | 99.9 | 79.7 |
| Particulate fraction | 3.4 | 39.7 | <0.1 | 20.3 | |
| Osmotic shock | Extracellular shock fluid | 1.8 | 8.7 | <0.1 | 2.3 |
| Soluble (cytoplasmic) fraction of spheroplasts | 87.0 | 72.5 | 57.2 | 77.4 | |
| Particulate fraction of spheroplasts | 2.6 | 19.7 | 18.5 | 13.1 | |
| Total recoveryb | 91.4 | 100.9 | 75.7 | 92.8 | |
a
The total amounts in 1010 sonicated C. xylanolytica cells were as follows: ArfI, 0.36 U; ENDOX, 0.36 U; GPD, 0.66 U; and protein, 1.33 mg.
b
Based on values given in footnote a.