TABLE 1.
Effects of detergents on extraction of cell-bound PUR esterase
| Detergent | PUR esterase activity (U/ml) |
|---|---|
| None | 0.0 |
| CHAPSa | 3.5 |
| CHAPSOb | 3.5 |
| BIGCHAP | 2.1 |
| deoxy-BIGCHAP | 6.6 |
| n-Octyl-β-d-glucoside | 1.2 |
| n-Heptyl-β-d-thioglucoside | 0.9 |
| n-Octyl-β-d-thioglucoside | 0.9 |
| n-Dodecyl-β-d-maltoside | 5.8 |
| MEGA-8c | 0.9 |
| MEGA-9d | 0.9 |
| MEGA-10e | 2.6 |
| Sucrose monocaprate | 0.9 |
| Sucrose monolaurate | 2.6 |
| Sodium cholate | 1.6 |
| Digitonin | 3.3 |
| Total cell-bound esterase activity | 5.7 |
a
CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate.
b
CHAPSO, 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxypropanesulfonic acid.
c
MEGA-8, n-octanoyl-N-methylglucamide.
d
MEGA-9, n-nonanoyl-N-methylglucamide.
e
MEGA-10, n-decanoyl-N-methylglucamide.