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. 1998 Jan;64(1):62–67. doi: 10.1128/aem.64.1.62-67.1998

TABLE 2.

Purification of PUR esterasea

Step Vol (ml) Total protein (mg) Total activity (U) Sp act (U/mg) Fold Recovery (%)
Crude enzyme 500 1,500 3,300 2.2 1 100
(NH4)2SO4 precipitation 400 600 2,460 4.4 2.0 80
Phenyl-TOYOPEARL 650M 300 135 1,560 11.6 5.3 47
Q-Sepharose FF 156 46 840 18.3 8.3 25
a

Enzyme activity was measured by using p-nitrophenyl acetate as a substrate.