TABLE 2.
Shake flask extents of TCE degradation by Tom after 18 h, shake flask initial TCE degradation rates (first 5 min, 10 μM TCE), specific growth rates, and root colonization in nonmicrocosm studies
| Sample | % of TCE removeda | Initial TCE degradation rate (nmol/min · mg of cells) | Specific growth rate in LB medium (h−1) | Root colonization (106, CFU/cm of root) |
|---|---|---|---|---|
| P. fluorescens 2-79 (negative control, no tom) | 2 | 0.0 | 0.77 | 4 |
| P. fluorescens 2-79TOM (constitutive Tom) | 97 | 1.1 | 0.78 | 3 |
| P. fluorescens 2-79TOM-S (uninduced, no salicylate) | 14 | 0.0 | NDb | ND |
| P. fluorescens 2-79TOM-S + 2 mM salicylate | 99 | 2.6 | 0.77 | 3 |
| B. cepacia PR123(TOM23C) (constitutive, positive control, non-root colonizer) | 98 | 2.5 | 0.86 | 0.04 |
The initial TCE concentration was 10 μM.
ND, not determined.