TABLE 3.
TCE microcosm data and parameters
Samplea | Extent of TCE degradation (% of TCE removed) |
Avg TCE degradation (% of TCE removed) |
Length of expt (days) | Root colonization (105, CFU/cm of root) |
Initial degradation rate (nmol/day · plant)b |
Length of plant growth before TCE was added (days) |
No. of seeds |
---|---|---|---|---|---|---|---|
Uninoculated microcosm 1 | 19 | 9 ± 8c | 12 | NAd | 0 | 5 | 100 |
Uninoculated microcosm 2 | 4 | 9 | NA | 2 | 5 | 140 | |
Uninoculated microcosm 3 | 5 | 6 | NA | 2 | 7 | 130 | |
Sterile soil microcosm 1 | 3 | 4 ± 1 | 6 | NA | 0 | NA | NA |
Sterile soil microcosm 2 | 5 | 6 | NA | 0 | NA | NA | |
2-79 microcosm 1 | 26 | 13 ± 7 | 11 | NDe | 7 | 7 | 110 |
2-79 microcosm 2 | 7 | 6 | 7 ± 2 | 1 | 7 | 160 | |
2-79 microcosm 3 | 16 | 7 | 16 ± 8 | 5 | 7 | 160 | |
2-79 microcosm 4 | 15 | 6 | 11 ± 4 | 2 | 7 | 160 | |
2-79 microcosm 5 | 6 | 6 | 6 ± 5 | 1 | 7 | 160 | |
2-79 microcosm 6 | 10 | 6 | 5 ± 2 | 0 | 7 | 160 | |
2-79TOM microcosm 1 | 53 | 63 ± 11 | 6 | ND | 12 | 8 | 150 |
2-79TOM microcosm 2 | 67 | 6 | ND | 22 | 8 | 150 | |
2-79TOM microcosm 3 | 85 | 6 | ND | 39 | 8 | 150 | |
2-79TOM microcosm 4 | 48 | 7 | 0.7 ± 0.03 | 15 | 6 | 160 | |
2-79TOM microcosm 5 | 65 | 7 | 11 ± 9 | 26 | 6 | 160 | |
2-79TOM microcosm 6 | 63 | 7 | 2 ± 1 | 24 | 6 | 160 | |
2-79TOM microcosm 7 | 62 | 7 | 10 ± 2 | 15 | 6 | 175 | |
2-79TOM microcosm 8 | 71 | 7 | 9 ± 4 | 14 | 6 | 175 | |
2-79TOM microcosm 9 | 55 | 7 | 5 ± 4 | 19 | 6 | 175 |
Uninoculated microcosms contained sterilized soil and wheat; 2-79 microcosms contained sterilized soil, wheat, and P. fluorescens 2-79; and 2-79TOM microcosms contained sterilized soil, wheat, and P. fluorescens 2-79TOM. The initial TCE concentration in all microcosms was 128 μM (assuming that all of the TCE was in 50 ml of water in each microcosm jar).
The initial TCE degradation rate was determined by determining the difference between the TCE concentrations in the headspace obtained 3 and 24 h after TCE was added.
Mean ± standard deviation.
NA, not applicable.
ND, not determined.