Figure 5. The Dependence of the Rate of Deamination of Adenosine upon Substrate Concentration with 17 nM Q8IJA9_PLAFA.

The open circles are individual data points, while the solid line is the fit of the data to Equation 1. The inset shows raw data for the deamination of three substrates by Q8IJA9_PLAFA as detected by loss of absorbance at 265 nm. The bold, thin, and dashed lines are data for 100 μM adenine, AMP, and adenosine, respectively. The reactions with adenine and AMP contained 860 nM enzyme, while the assay containing adenosine had only 17 nM enzyme. Reaction conditions for all assays were 25 °C in 50 mM potassium phosphate (pH 7.4).