FIG. 4.

Effects of disruption of A. polyphaga microtubules (by colchicine) and microfilament (by cytochalasin D) and inhibition of protein synthesis (by cycloheximide) on invasion by L. pneumophila. The percent invasion was determined by dividing the number of intracellular bacteria recovered following the invasion period after killing of extracellular bacteria by the number of intracellular bacteria in untreated samples. Values are the means of triplicate samples, and error bars represent standard deviations.