FIG. 1.

Effects of growth temperature on expression of the 40-kDa MOMP. V. anguillarum O2 cultures were grown in CM9 for 16 h at the indicated temperatures, and cell suspensions were adjusted to an OD₅₂₀ of 1 (1 × 10⁹ to 3 × 10⁹ CFU/ml). The cell pellet from 1 ml of the suspension was lysed in 60 μl of sample buffer (with 2-ME and SDS) and heated for 10 min at 100°C, and 10-μl samples were loaded in each lane. (A) Coomassie blue-stained gel; (B) blot probed with monospecific MOMP-Ab. The arrowheads indicate the positions of a novel 60-kDa protein and the MOMP. Molecular mass standards are indicated on the left.