FIG. 3.

Expression of the 40-kDa MOMP is enhanced by increased medium osmolarity and salt concentration. V. anguillarum O2 cell cultures were grown at 25°C in CM9 medium containing increasing concentrations of NaCl (A) and sucrose (B and C). Cell lysates for SDS-PAGE were prepared as described in the legend to Fig. 1. (A and C) Blots probed with monospecific MOMP-Ab. (B) SDS-PAGE of lysates of cells grown in increasing sucrose concentrations. Molecular mass standards are indicated on the left.