FIG. 5.

Transition from monomer to trimer of the V. anguillarum O2 MOMP is dependent on temperature and SDS. Purified MOMP (4 μg of protein/lane) was mixed with sample buffer containing the indicated combinations of SDS and 2-ME. SDS-PAGE of MOMP samples treated at 100°C for 10 min (A) and of MOMP samples treated at the indicated temperatures for 10 min in sample buffer containing SDS and 2-ME (B) and 2-ME without added SDS (C) is shown. The apparent molecular masses of the MOMP conformational forms are shown on the right. Molecular mass standards are indicated on the left.