TABLE 2.

Effects of environmental conditions on the growth and viability of V. anguillarum O2^a

Condition	OD520	CFU/ml
Temp (°C)
15	0.25	3.69 × 106
18	0.29	3.86 × 106
25	0.52	3.2 × 107
37	0.15	1.5 × 105
EDTA (mM)
0	0.72	9.5 × 108
0.1	0.80	1.9 × 109
0.3	0.70	2.49 × 108
0.6	0.69	6.0 × 107
1.0	0.67	5.5 × 107
5.0	0.06	2.38 × 104
NaCl (mM)
0	0.54	4.1 × 107
50	0.71	1.77 × 108
85.6	0.81	1.06 × 108
100	0.87	1.62 × 108
200	0.56	7.2 × 107
400	0.57	3.6 × 107
500	0.46	9.4 × 107
600	0.16	1.25 × 105
800	0.015	NDb
Sucrose (%)
0.4	0.664	7.5 × 107
1.0	0.753	1.17 × 108
2.0	0.849	4.1 × 108
5.0	0.990	4.0 × 108
7.5	1.072	4.6 × 108
10.0	1.200	3.4 × 108

^aAll cultures were initially inoculated with 9 × 10⁴ to 9.5 × 10⁴ CFU/ml. OD₅₂₀ and CFU per milliliter were determined following growth for 16 h (for temperature), 24 h (for sucrose and NaCl), or 48 h (for EDTA). 

^bND, not determined.