FIG. 2.

(A) Kinetics of morpholine degradation by M. aurum MO1. Resting cells (5 g of wet cells in 50 ml of Knapp buffer [KH₂PO₄, 1 g · liter⁻¹; K₂HPO₄, 1 g · liter⁻¹; FeCl₃, 4 mg · liter⁻¹; MgSO₄ · 7H₂O, 40 mg · liter⁻¹; pH 6.6]) were incubated with 10 mM morpholine at 30°C with agitation (200 rpm) for 72 h. Samples (1 ml) were collected every hour for 12 h and from time to time until 72 h; after centrifugation, the supernatants of these samples were analyzed by ¹H-NMR spectroscopy at 300.13 MHz. TSPd₄ was used as a reference for chemical shifts and quantification. (B). Expanded scale, from 2.60 to 3.98 ppm, of the 10-h spectrum. M, morpholine; G, glycolate; Y, 2-(2-aminoethoxy)acetate.