FIG. 3.

Kinetics of morpholine degradation by Mycobacterium sp. strain RP1. Resting cells (5 g of wet cells in 50 ml of Knapp buffer) were incubated with 10 mM morpholine at 30°C with agitation (200 rpm) for 72 h. Samples (1 ml) were collected every hour for 12 hours and from time to time until 72 h; after centrifugation, the supernatants of these samples were analyzed by ¹H NMR spectroscopy at 300.13 MHz. TSPd₄ was used as a reference for chemical shifts and quantification. The inset corresponds to an expanded scale, from 2.60 to 4.00 ppm, of the 20-h spectrum. M, morpholine; Y, 2-(2-aminoethoxy)acetate; G, glycolic acid.