FIG. 3.

SDS-PAGE of MT-I. Pooled fractions following elution of MT-I were analyzed by SDS-PAGE on a 13% polyacrylamide gel, and polypeptide bands were stained with Coomassie brilliant blue R-250. Lane 1, pooled fraction after first phenyl-Sepharose fractionation; lane 2, pooled fraction after DEAE-Sepharose fractionation; lane 3, MT-I after second phenyl-Sepharose fractionation; lane 4, MT-I after Sephacryl S-300 pool; lane 5, flowthrough fraction (pure MT-I) from Matrex gel Green A. Molecular mass standards (Pharmacia LKB) were bovine serum albumin (67 kDa), ovalbumin (43 kDa), carbonic anhydrase (30 kDa), soybean trypsin inhibitor (20.1 kDa), and α-lactalbumin (14.4 kDa).