Conversion of toluene (A) and TCE (B) by the fed-batch culture. The concentrations of toluene and TCE in the ingoing and outgoing gas streams were determined by gas chromatography. The chloride concentration in the culture medium was determined with a colorimetric assay. Symbols: •, toluene in the ingoing gas stream; ○, toluene in the outgoing gas stream; ◊, percentage of toluene degraded by the culture; ▪, TCE in the ingoing gas stream; □, TCE in the outgoing gas stream; ⧫, percentage of TCE degraded by the culture (calculated from the concentrations of TCE in the in- and outgoing gas streams); ▴, chloride concentration; ▵, percentage of TCE degraded by the fed-batch culture (calculated from chloride measurements); ↓, time at which TCE addition was started.