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Analysis of TRI11 gene disruptants in F. sporotrichioides by PCR. Transformant and NRRL 3299 DNA preparations were used as templates for PCRs primed with oligonucleotides 677 and 678. PCR products were separated on a 1% agarose gel (40 mM Tris-acetate, 1 mM EDTA) and stained with ethidium bromide. Lanes 1 to 5, PCR products from five different transformant DNAs; lane 6, PCR product from F. sporotrichioides NRRL 3299 DNA.
