TABLE 1.

Influence of 3 M NaCl on accumulation of GB and COS in P. fellutanum

LPSG medium supplement	Solute (mg/g [dry wt])a		Mycelium growth (g/200 ml)b
	COS	GB
None	9.3 (17)c	1.5 (3.0)	3.5 ± 0.35
NaCld	25.0 (46)	33.1 (70)	1.8 ± 0.01

^aThe quantity of each solute is provided as milligrams of COS or GB per gram (dry weight) of mycelium. The quantity of each solute was determined by integrating methyl carbon signals on ¹³C NMR spectra by using Felix for Windows 1.02 software (Molecular Simulations Inc.). Sodium TSP (0.51%) was used as a reference, and the data were acquired without nuclear overhauser effect enhancement. 

^bCultures were grown for 8 days in 200 ml each of LPSG medium in three separate flasks with 3 M NaCl and without added NaCl as a control. Growth was determined by measuring mycelium dry weight and by determining the means of the results of triplicate experiments ± standard deviations in grams (dry weight) per 200 ml of culture medium. 

^cValues in parentheses represent the calculated millimolar concentration of the indicated solute. An approximate cell composition of 20% dry weight–80% water was assumed. 

^dSterile, solid NaCl (35.06 g) was added to 200 ml of culture.