TABLE 2.
Effect of exogenous GB or COS on growtha of P. fellutanum stressed with 3 M NaCl
Day | Mycelium growth (mg [dry wt])
|
||
---|---|---|---|
Control | GBb | COSb | |
1 | 9 | ||
2 | 30 | ||
2.5d | 60 | ||
3 | 40 | 60 (50)c | 75 (88) |
4 | 70 | 90 (29) | —e |
6 | 110 | 110 (27) | 120 (9) |
8 | 140 | 200 (43) | 180 (29) |
P. fellutanum was grown with or without added GB (2 mM) or COS (10 mM) in LPSG medium supplemented with 3 M NaCl. The growth was assessed by measuring the mycelium dry weight and determining the mean values of the results for duplicate samples. Dry weight was determined as described previously (39). The difference in dry weight between duplicate samples was less than 10%.
GB and COS were dissolved in ddH2O separately and filtered through a 0.22-μm-pore-size membrane.
Values in parentheses represent percentage increases as related to milligrams (dry weight) of mycelium in cultures with added GB or COS compared with that of control set at 100% on same day.
Time of addition of sterile solid NaCl; the final concentration of NaCl was 3 M. GB and COS were added 30 min before the addition of NaCl.
—, not measured.