FIG. 3.

Phylogenetic positions of representative OCS and OM rDNA clones related to the chlorophyte line of descent. This phylogenetic tree was constructed by the maximum-likelihood method (fastDNAml [43]). Bootstrap values (100 replicates) from both neighbor-joining (above each node) and maximum-parsimony (below each node) analyses are shown. A conserved mask of 1,133 nucleotides (nucl.), spanning the entire length of the plastid SSU rRNA gene, was included. The gene sequences of Cyanophora paradoxa, Ochrosphaera neapolitana, E. huxleyi, and an Isochrysis sp. were used to root the phylogenetic tree. All the sequences used, except for those of Closterium ehrenbergii, Chlamydomonas parkeae, Yamagishiella unicocca, Gonium pectorale, and P. parkeae (30), are available from the GenBank or RDP database. cp, chloroplast.