TABLE 3.
Effect of solution upon adsorption of E. coli O157KB0 Rifr and V9 Nalr in the mixed cell suspension to Dynabeads (M280; coated with sheep anti-rabbit IgG)
| Before adsorption (cells/ml ± SD)
|
Solution used for:
|
No. of cells adsorbed (cells/ml ± SD)
|
|||||
|---|---|---|---|---|---|---|---|
| Rifampin selection | Nalidixic acid selection | Ratio of cells (Rifr:Nalr) | Adsorption | Washing | Rifampin selection | Nalidixic acid selection | Ratio of cells (Rifr:Nalr) |
| (2.7 ± 1.1) × 105 | (5.5 ± 0.3) × 108 | 4.9 × 10−4:1 | BHI | BHI | 5 ± 10 | (4.5 ± 1.1) × 104 | 1.1 × 10−4:1 |
| CMQ | CMQ | (1.5 ± 0.4) × 103 | (8.9 ± 2.2) × 102 | 1.7:1 | |||