TABLE 7.
Serotype examination of the E. coli-like coloniesa grown on MacConkey agar plate after IMS
| Expt no. | Solution used for:
|
No. of O157 antigen-positive and rifampin-resistant colonies | No. of O157 antigen-negative colonies
|
Recovered O157 colonies (%) | ||
|---|---|---|---|---|---|---|
| IMS | Washing | Nalidixic acid resistant | Rifampin sensitive | |||
| A | BPW | PBS-Tween 20 | 15 | 2 | 8 | 60 |
| CMQ | CMQ | 23 | 0 | 2 | 92 | |
| B | BPW | PBS-Tween 20 | 20 | 1 | 4 | 80 |
| CMQ | CMQ | 24 | 0 | 1 | 96 | |
| C | BPW | PBS-Tween 20 | 8 | 11 | 6b | 32 |
| CMQ | CMQ | 25 | 0 | 0 | 100 | |
| D | BPW | PBS-Tween 20 | 14 | 11 | 0 | 56 |
| CMQ | CMQ | 24 | 1 | 0 | 96 | |
| E | BPW | PBS-Tween 20 | 3 | 22 | 0 | 12 |
| CMQ | CMQ | 22 | 3 | 0 | 88 | |
a
A total of 25 colonies were examined.
b
All six colonies were H. alvei.