Table 4.
Immature rat uterotrophic assays (4 days’ duration) using synthetic diets of different ME content (experiment 4).
| Treatment | Diet ME intake (kJ/g diet)a | Total energy intake (kJ)b/rat | Absolute uterine blotted weight (mg)c | Adjusted uterine blotted weight (mg)d | Final body weight (g)c | No. |
|---|---|---|---|---|---|---|
| RM1/AO | 10.9 | 222 | 21.4 ± 3.2 | 22.4 | 51.2 ± 7.2 | 10 |
| Diet B/AO | 12.1 | 243 | 29.2 ± 7.4** | 30.7** | 50.0 ± 8.0 | 10 |
| AIN-76A/AO | 15.7 | 325 | 35.8 ± 6.4** | 34.9** | 55.8 ± 8.0** | 10 |
| Diet C/AO | 16.2 | 316 | 34.7 ± 9.1** | 34.5** | 54.0 ± 7.8** | 10 |
| Diet D/AO | 20.3 | 218 | 34.4 ± 5.6** | 32.5** | 58.2 ± 7.6** | 10 |
| RM1/DES 5 μg/kg | 10.9 | 434 | 105.1 ± 3.4** | 106.1** | 51.3 ± 6.6 | 4 |
| RM1/ANT | 10.9 | 222 | 17.1 ± 1.9 | 18.5 | 50.0 ± 6.8 | 10 |
| Diet B/ANT | 12.1 | 227 | 17.3 ± 2.4 | 18.8 | 49.8 ± 7.8 | 10 |
| AIN-76A/ANT | 15.7 | 336 | 17.8 ± 1.2 | 17.0 | 55.6 ± 6.4# | 10 |
| Diet C/ANT | 16.2 | 314 | 18.1 ± 2.0 | 18.3 | 53.2 ± 8.3# | 10 |
| Diet D/ANT | 20.3 | 422 | 18.8 ± 2.2 | 16.9 | 58.3 ± 6.5# | 10 |
| RM1/DES 5 μg/kg/ANT | 10.9 | 232 | 119.2 ± 7.3# | 120.6# | 49.6 ± 7.5 | 4 |
ND, not determined. DES was administered sc.
a
The ME value for RM1 was taken from the manufacturer’s data sheet.
b
Total energy intake was calculated as the product of the total amount of food consumed per rat over the duration of the study and the ME of the diet.
c
Mean ± SD.
d
Uterine weights adjusted for covariance with terminal body weights.
**p < 0.01 compared with RM1 or RM1/AO control.
#
p < 0.01 compared with RM1/ANT control.