KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Paraformaldehyde | Electron Microscopy Sciences | Cat# 15710; CAS: 30525-89-4 |
| PBS, pH 7.4 | Quality Biological | Cat# 114-058-131 |
| Triton X-100 | VWR Life Science | Cat# 0694-1L; CAS: 9036-19-5 |
| Tris-HCl, 1M, pH 8.0 | Quality Biological | Cat# 351-007-101 |
| SDS, 20% | Quality Biological | Cat# 351-066-101; CAS: 151-21-3 |
| NaCl, 5M | KD Medical | Cat# RGE-3270; CAS: 7647-14-5 |
| EDTA, 0.5M | KD Medical | Cat# RGC-3130; CAS: 60-00-4 |
| Sodium deoxycholate | Sigma | Cat# D6750; CAS: 302-95-4 |
| Tween-20 | VWR Life Science | Cat# 0777-1L CAS: 9005-64-5 |
| Formamide (Deionized) | Invitrogen | Cat# AM9342; CAS: 75-12-7 |
| Dextran Sulfate Sodium Salt | Thermo Scientific | Cat# J14489-09; CAS: 9011-18-1 |
| Aphidicolin | MedChem Express | Cat# HY-N6733; CAS: 38966-21-1 |
| Vectashield mounting medium with DAPI | Vector Laboratories | Cat# H-1200-10 |
| Vectashield mounting medium | Vector Laboratories | Cat# H-1000-10 |
| FBS | Gibco | Cat# A5670701 |
| Penicillin–streptomycin | Gibco | Cat# 15140122 |
| BSA | Sigma | Cat# A8806 |
| Schneider’s Drosophila medium | Gibco | Cat# 21720 |
| XhoI | New England Biolabs | Cat# R0146L |
| BglII | New England Biolabs | Cat# R0144M |
| Hi T4 DNA ligase | New England Biolabs | Cat# M2622L |
| T4 DNA ligase | New England Biolabs | Cat# M0202L |
| Lambda exonuclease | New England Biolabs | Cat# M0262L |
| 20×SSC buffer | KD Medical | Cat# RGF-3240 |
| Salmon sperm DNA | Invitrogen | Cat# 15-632-011 |
| dNTP Solution Set | New England Biolabs | Cat# N0446S |
| Agarose | Lonza Bioscience | Cat# 50004 |
| TAE Buffer | Quality Biological | Cat# 351-008-491 |
| CF®405M Phalloidin | Biotium Inc | Cat# 00034 |
| Critical commercial assays | ||
| EquiPhi29™ DNA Polymerase | Thermo Scientific | Cat# A39391 |
| Click-iT™ EdU Cell Proliferation Kit | Invitrogen | Cat# C10337 |
| DNeasy Blood & Tissue kit | QIAGEN | Cat# 69504 |
| Taq DNA Polymerase High Fidelity | Invitrogen | Cat# 11304029 |
| Experimental models: Organisms/strains | ||
| D. melanogaster: TFAM-mNeonGreen | Zhang et al.33 | N/A |
| D. melanogaster: w1118 (mt:ND2ins) | Xu et al.26 | N/A |
| D. melanogaster: w1118 (mt:CoIT300I) | Xu et al.26 | N/A |
| D. melanogaster: hetero-1.1 | This paper | N/A |
| D. melanogaster: hetero-4.3 | This paper | N/A |
| D. melanogaster: hetero-4.4 | This paper | N/A |
| Oligonucleotides | ||
| XhoI padlock probes: 5’Phos/CAACGGTAAATATATGCAACAAACTGGTGAAAGTCGCTATCCCGTTCAGGAAAAGTATCTACATCTATTC | This paper | N/A |
| BglII padlock probes: 5Phos/ATGATTAATTGAAGAAACGAAGTTTTCATAAGACACGGAGAGAGAGCACCAAAATTAATATTCACCCTAA | This paper | N/A |
| XhoI detection probes: 5’Cy5/CTGGTGAAAGTCGCTATCCCGTT | This paper | N/A |
| XhoI detection probes: 5’Alexa488/CTGGTGAAAGTCGCTATCCCGTT | This paper | N/A |
| XhoI detection probes: 5’Cy3/CTGGTGAAAGTCGCTATCCCGTT | This paper | N/A |
| BglII detection probes: 5’Cy3/TTCATAAGACACGGAGAGAGAG | This paper | N/A |
| PCR primers for quantifying mt:CoIT300I load TGGAGCTATTGGAGGACTAAATCAGCTCCTGTTAATGGTCATGGACT | Hill et al.8 | N/A |
| Software and algorithms | ||
| Prism | GraphPad | https://www.graphpad.com/ |
| Fiji | NIH | https://fiji.sc; https://doi.org/10.1186/s12859-017-1934-z |
| Imaris | Oxford Instruments | https://imaris.oxinst.com |
| Deposited data | ||
| Original microscopy images and Excel files | This paper; Mendeley Data | DOI: 10.17632/vtbvbwwfvt.1 |
| Other | ||
| Nikon CSU-W1 SoRa confocal system | Nikon | N/A |
| GelDoc Go Gel Imaging System | BIO-RAD | N/A |