Table 4.

Sequences of siRNAs used for in vivo study and T_m values

siRNA	Modification at position 7 of antisense strand	Sequences (5′ -3′)a	T m [°C]b	ΔTm [°C]c
si9	-	a•g•uguuCuUGCucuauaaaca*	87.27	-
		u•G•uuuauagagcaAgAacacu•g•u
si10	UNA	a•g•uguuCuUGCucuauaaaca*	81.97	-5.30
		u•G•uuuaUagagcaAgAacacu•g•u
si11	5′-(S)-Me-UNA	a•g•uguuCuUGCucuauaaaca*	81.97	-5.30
		u•G•uuuaU5′SagagcaAgAacacu•g•u
si12	5′-(R)-Me-UNA	a•g•uguuCuUGCucuauaaaca*	82.02	-5.25
		u•G•uuuaU5′RagagcaAgAacacu•g•u
si13	2′-(S)-Me-UNA	a•g•uguuCuUGCucuauaaaca*	83.02	-4.25
		u•G•uuuaU2′SagagcaAgAacacu•g•u
si14	2′-(R)-Me-UNA	a•g•uguuCuUGCucuauaaaca*	82.12	-5.15
		u•G•uuuaU2′R agagcaAgAacacu•g•u
si15	3′-(S)-Me-UNA	a•g•uguuCuUGCucuauaaaca*	81.97	-5.30
		u•G•uuuaU3′S agagcaAgAacacu•g•u
si16	3′-(R)-Me-UNA	a•g•uguuCuUGCucuauaaaca*	81.97	-5.30
		u•G•uuuaU3′R agagcaAgAacacu•g•u
si17	4′-(β)-OMe-UNA	a•g•uguuCuUGCucuauaaaca*	82.97	-4.30
		u•G•uuuaU4′bagagcaAgAacacu•g•u

^aUppercase letters, 2′-F nucleotides; lowercase letters, 2′-OMe nucleotides; U, UNA; U_5′S, 5′-(S)-Me-UNA; U_5′R, 5′-(R)-Me-UNA; U_2′S, 2′-(S)-Me-UNA; U_2′R, 2′-(R)-Me-UNA; U_3′S, 3′-(S)-Me-UNA; U_3′R, 3′-(R)-Me-UNA; U_4′β, 4′-(β)-methoxy-UNA;• phosphorothioate linkages; *, trivalent GalNAc ligand.

^bThe absorbances of hybridized duplexes (2.5 μM) at 260 nm were determined as a function of temperature in PBS. The T_m was determined as the maximum of the first derivative of the melting curve. Values are reported as the average of two independent experiments.

^cΔT_m was calculated with respect to si9.