TABLE 1.
Comparative effect of LPS and poly(I:C) on the expression of MHC class II and B7.1 on phagocytic ability and T-cell priming ability of feline DCs
| Stimulus (amt) | MFIa
|
cpmb(mean ± SE) | ||
|---|---|---|---|---|
| MHC class II | B7.1 | FITC-DXc | ||
| None | 20 | 3 | 34 | 1,352 ± 275 |
| LPS (50 ng/ml) | 362 | 25 | 21 | 22,013 ± 1,268 |
| Poly(I:C) (20 μg/ml) | 82 | 14 | 16 | 2,344 ± 654 |
a
Mean fluorescence intensity (MFI) of each population subtracted from MFI of background (see Materials and Methods). One representative experiment of five is shown.
b
DCs (104) were incubated for 5 days with 105 allogeneic PBMCs, and then [methyl-3H]thymidine was added to wells for the last 18 h. Results are expressed as the means ± standard errors in cpm of triplicates.
c
FITC-DX was added to DCs at 200 μg/ml, and DCs were incubated for 45 min at 37°C or on ice (background).