TABLE 2.
Results of PCR assay of spirochetal isolates from Missouri and Georgia with six B. burgdorferi-specific primer pairs
| Strain, date of isolation | Tick | Reactivities of the indicated primer pairs with the following gene sequences:
|
|||||
|---|---|---|---|---|---|---|---|
|
ospA
|
fla (245-855) | Chromosomal (147-520) | |||||
| 788-946 | 149-319 | 149-459 | 3′-5′ | ||||
| MOD-1, September 1993 | I. dentatus, two nymphs | + | + | + | + | + | − |
| MOD-2, September 1993 | I. dentatus, two nymphs | − | + | + | + | + | − |
| MOD-3, September 1993 | I. dentatus, four larvae | − | + | + | + | + | − |
| MOD-5, November 1994 | I. dentatus, one nymph | + | + | + | + | + | + |
| MOD-6, October 1995 | A. americanum, one larva | − | + | + | + | + | − |
| BC-1, February 1993 | I. dentatus, one nymph | − | + | + | + | + | − |
| B-31, reference strain | I. scapularis | + | + | + | + | + | + |