Fig. 1.

Cloning and mapping p600. (A) Silver staining of the purified RB complex. e-RB was purified from e-RB-expressing HeLa cells by immunoprecipitation with anti-FLAG antibody (lane 2). As a control, mock purification was performed with nontransduced HeLa cells (lane 1). (B) p600 is a calmodulin-binding protein. The purified RB complex (lanes 2 and 4) and nontransduced control (lanes 1 and 3) were incubated with biotinylated calmodulin in buffer containing CaCl₂ (lanes 1 and 2) or EGTA (lanes 3 and 4).