TABLE 1.
Summary of genotypic characterization results obtained in this study for cpe-positive C. perfringens isolates
Isolate group and source | Strain (reference) |
cpe RFLP pattern (kb) obtained with the follow- ing endonuclease:
|
PFGE-determined cpe locationa | |
---|---|---|---|---|
EcoRI | NruI | |||
cpe-negative strain | ATCC 3624 (19) | Negative | Negative | Negative |
Food-borne disease isolatesb | ||||
1950s European food-poisoning isolates | NCTC 8235 (19) | 10 | 5 | ND |
NCTC 8238 (19) | 10 | 5 | ND | |
NCTC 8239 (19) | 10 | 5 | S | |
NCTC 8359 (19) | 10 | 5 | ND | |
NCTC 8798 (19) | 10 | 5 | ND | |
NCTC 8799 (19) | 10 | 5 | ND | |
NCTC 10239 (19) | 10 | 5 | Chromosomal | |
1980s North American food-poisoning isolates | C-1841 (3) | 10 | 5 | Chromosomal |
C-1849 (3) | 10 | 5 | ND | |
C-1851 (3) | 10 | 5 | ND | |
C-1869 (3) | 10 | 5 | ND | |
C-1881 (3) | 10 | 5 | ND | |
C-1887 (3) | 10 | 5 | ND | |
FD 1041 (R. Labbe) | 10 | 5 | Chromosomal | |
1990s North American food-poisoning isolate | 5 (19) | 10 | 5 | ND |
Non-food-borne disease isolates | ||||
Stool isolates from 1980s European antibioticassociated diarrhea studyc | B2 (22) | >20 | >20 | Episomal |
B11 (22) | >20 | >20 | Episomal | |
B38 (22) | >20 | >20 | Episomal | |
B41 (22) | >20 | >20 | ND | |
B45 (22) | >20 | >20 | ND | |
Newbury 16 (P. Borriello) | >20 | >20 | Episomal | |
Stool isolates from 1990s European SPOR studyd | F4013 (M. Brett) | >20 | >20 | ND |
F4129 (M. Brett) | >20 | >20 | Episomal | |
F4393 (M. Brett) | >20 | >20 | ND | |
F4396 (M. Brett) | >20 | >20 | ND | |
F4406 (M. Brett) | >20 | >20 | ND | |
F4591 (M. Brett) | >20 | >20 | Episomal | |
F4859 (M. Brett) | >20 | >20 | ND | |
F4969 (M. Brett) | >20 | >20 | Episomal | |
F5537 (M. Brett) | >20 | >20 | ND | |
F5599 (M. Brett) | >20 | >20 | ND | |
F5603 (M. Brett) | >20 | >20 | Episomal | |
Veterinary isolates | ||||
1990s European veterinary isolate from pig with enteritis | 29e (D. J. Taylor) | 10 | 5 | S |
1990s North American veterinary isolates | 153e (19) | >20 | >20 | ND |
157 (19) | >20 | >20 | ND | |
222e (19) | >20 | >20 | Episomal | |
382 (19) | >20 | >20 | ND | |
452e (19) | >20 | >20 | Episomal | |
455e (19) | >20 | >20 | ND | |
456e (19) | >20 | >20 | ND | |
457e (19) | >20 | >20 | ND | |
458e (19) | >20 | >20 | Episomal |
ND, not determined; S, smearing of DNA bands observed (see Results).
Note that in reference 19, NCTC 8235 is called 182, NCTC 8238 is called 176, NCTC 8359 is called 175, and NCTC 8799 is called 183.
Note that these isolates, belonging to several different capsular serotypes, were all obtained from CPE-positive feces of diarrheic humans.
Note that these isolates were all obtained form CPE-positive feces of humans suffering from isolated cases of sporadic diarrhea.
Note that these isolates were obtained form diarrheic domestic animals.