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. 1998 Jan;36(1):30–36. doi: 10.1128/jcm.36.1.30-36.1998

TABLE 1.

Summary of genotypic characterization results obtained in this study for cpe-positive C. perfringens isolates

Isolate group and source Strain (reference) cpe RFLP pattern (kb) obtained with the follow- ing endonuclease:
PFGE-determined cpe locationa
EcoRI NruI
cpe-negative strain ATCC 3624 (19) Negative Negative Negative
Food-borne disease isolatesb
 1950s European food-poisoning isolates NCTC 8235 (19) 10 5 ND
NCTC 8238 (19) 10 5 ND
NCTC 8239 (19) 10 5 S
NCTC 8359 (19) 10 5 ND
NCTC 8798 (19) 10 5 ND
NCTC 8799 (19) 10 5 ND
NCTC 10239 (19) 10 5 Chromosomal
1980s North American food-poisoning isolates C-1841 (3) 10 5 Chromosomal
C-1849 (3) 10 5 ND
C-1851 (3) 10 5 ND
C-1869 (3) 10 5 ND
C-1881 (3) 10 5 ND
C-1887 (3) 10 5 ND
FD 1041 (R. Labbe) 10 5 Chromosomal
1990s North American food-poisoning isolate 5 (19) 10 5 ND
Non-food-borne disease isolates
 Stool isolates from 1980s European antibioticassociated diarrhea studyc B2 (22) >20 >20 Episomal
B11 (22) >20 >20 Episomal
B38 (22) >20 >20 Episomal
B41 (22) >20 >20 ND
B45 (22) >20 >20 ND
Newbury 16 (P. Borriello) >20 >20 Episomal
 Stool isolates from 1990s European SPOR studyd F4013 (M. Brett) >20 >20 ND
F4129 (M. Brett) >20 >20 Episomal
F4393 (M. Brett) >20 >20 ND
F4396 (M. Brett) >20 >20 ND
F4406 (M. Brett) >20 >20 ND
F4591 (M. Brett) >20 >20 Episomal
F4859 (M. Brett) >20 >20 ND
F4969 (M. Brett) >20 >20 Episomal
F5537 (M. Brett) >20 >20 ND
F5599 (M. Brett) >20 >20 ND
F5603 (M. Brett) >20 >20 Episomal
Veterinary isolates
 1990s European veterinary isolate from pig with enteritis 29e (D. J. Taylor) 10 5 S
1990s North American veterinary isolates 153e (19) >20 >20 ND
157 (19) >20 >20 ND
222e (19) >20 >20 Episomal
382 (19) >20 >20 ND
452e (19) >20 >20 Episomal
455e (19) >20 >20 ND
456e (19) >20 >20 ND
457e (19) >20 >20 ND
458e (19) >20 >20 Episomal
a

ND, not determined; S, smearing of DNA bands observed (see Results). 

b

Note that in reference 19, NCTC 8235 is called 182, NCTC 8238 is called 176, NCTC 8359 is called 175, and NCTC 8799 is called 183. 

c

Note that these isolates, belonging to several different capsular serotypes, were all obtained from CPE-positive feces of diarrheic humans. 

d

Note that these isolates were all obtained form CPE-positive feces of humans suffering from isolated cases of sporadic diarrhea. 

e

Note that these isolates were obtained form diarrheic domestic animals.