TABLE 1.
Summary of genotypic characterization results obtained in this study for cpe-positive C. perfringens isolates
| Isolate group and source | Strain (reference) |
cpe RFLP pattern (kb) obtained with the follow- ing endonuclease:
|
PFGE-determined cpe locationa | |
|---|---|---|---|---|
| EcoRI | NruI | |||
| cpe-negative strain | ATCC 3624 (19) | Negative | Negative | Negative |
| Food-borne disease isolatesb | ||||
| 1950s European food-poisoning isolates | NCTC 8235 (19) | 10 | 5 | ND |
| NCTC 8238 (19) | 10 | 5 | ND | |
| NCTC 8239 (19) | 10 | 5 | S | |
| NCTC 8359 (19) | 10 | 5 | ND | |
| NCTC 8798 (19) | 10 | 5 | ND | |
| NCTC 8799 (19) | 10 | 5 | ND | |
| NCTC 10239 (19) | 10 | 5 | Chromosomal | |
| 1980s North American food-poisoning isolates | C-1841 (3) | 10 | 5 | Chromosomal |
| C-1849 (3) | 10 | 5 | ND | |
| C-1851 (3) | 10 | 5 | ND | |
| C-1869 (3) | 10 | 5 | ND | |
| C-1881 (3) | 10 | 5 | ND | |
| C-1887 (3) | 10 | 5 | ND | |
| FD 1041 (R. Labbe) | 10 | 5 | Chromosomal | |
| 1990s North American food-poisoning isolate | 5 (19) | 10 | 5 | ND |
| Non-food-borne disease isolates | ||||
| Stool isolates from 1980s European antibioticassociated diarrhea studyc | B2 (22) | >20 | >20 | Episomal |
| B11 (22) | >20 | >20 | Episomal | |
| B38 (22) | >20 | >20 | Episomal | |
| B41 (22) | >20 | >20 | ND | |
| B45 (22) | >20 | >20 | ND | |
| Newbury 16 (P. Borriello) | >20 | >20 | Episomal | |
| Stool isolates from 1990s European SPOR studyd | F4013 (M. Brett) | >20 | >20 | ND |
| F4129 (M. Brett) | >20 | >20 | Episomal | |
| F4393 (M. Brett) | >20 | >20 | ND | |
| F4396 (M. Brett) | >20 | >20 | ND | |
| F4406 (M. Brett) | >20 | >20 | ND | |
| F4591 (M. Brett) | >20 | >20 | Episomal | |
| F4859 (M. Brett) | >20 | >20 | ND | |
| F4969 (M. Brett) | >20 | >20 | Episomal | |
| F5537 (M. Brett) | >20 | >20 | ND | |
| F5599 (M. Brett) | >20 | >20 | ND | |
| F5603 (M. Brett) | >20 | >20 | Episomal | |
| Veterinary isolates | ||||
| 1990s European veterinary isolate from pig with enteritis | 29e (D. J. Taylor) | 10 | 5 | S |
| 1990s North American veterinary isolates | 153e (19) | >20 | >20 | ND |
| 157 (19) | >20 | >20 | ND | |
| 222e (19) | >20 | >20 | Episomal | |
| 382 (19) | >20 | >20 | ND | |
| 452e (19) | >20 | >20 | Episomal | |
| 455e (19) | >20 | >20 | ND | |
| 456e (19) | >20 | >20 | ND | |
| 457e (19) | >20 | >20 | ND | |
| 458e (19) | >20 | >20 | Episomal | |
a
ND, not determined; S, smearing of DNA bands observed (see Results).
b
Note that in reference 19, NCTC 8235 is called 182, NCTC 8238 is called 176, NCTC 8359 is called 175, and NCTC 8799 is called 183.
c
Note that these isolates, belonging to several different capsular serotypes, were all obtained from CPE-positive feces of diarrheic humans.
d
Note that these isolates were all obtained form CPE-positive feces of humans suffering from isolated cases of sporadic diarrhea.
e
Note that these isolates were obtained form diarrheic domestic animals.