TABLE 2.
Comparative identification of various streptococcal strains
| Straina | Relevant characteristicsb | Species identification
|
sodAint accession no. | |
|---|---|---|---|---|
| ID 32 Strepc | sodAintd | |||
| S. agalactiae | ||||
| CIP 82.45 | Lancefield group B (ATCC 12403) | ND | S. agalactiae (98.6) | Z99178 |
| NEM318 | Lancefield group B | ND | S. agalactiae (97.9) | Z99179 |
| NEM1317 | Lancefield group B | ND | S. agalactiae (98.4) | Z99180 |
| Milleri group | ||||
| NEM1164 | Nontypeable, β-hemolytic | S. constellatus (99.6) | S. anginosus (97) | Z99181 |
| NEM1166 | Nontypeable, β-hemolytic | S. anginosus (99.8) | S. anginosus (97.4) | Z99182 |
| NEM1124 | Lancefield group F, β-hemolytic | S. constellatus (99.9) | S. constellatus (98.1) | Z99183 |
| NEM1162 | Lancefield group F, β-hemolytic | S. constellatus (99.6) | S. constellatus (99.1) | Z99184 |
| NEM1165 | Nontypeable | S. constellatus (93.1) | S. constellatus (98.9) | Z99185 |
| NEM1275 | Nontypeable | S. constellatus (63.9) | S. constellatus (99.1) | Z99186 |
| MG19 | Nontypeable | Milleri group | S. constellatus (99.1) | Z99187 |
| Mitis group | ||||
| CIP 103221 | ATCC 33399 | S. gordonii (?) | S. gordonii (95.9) | Z99189 |
| BM120 | Strain Challis | S. gordonii (99.9) | S. gordonii (98.2) | Z99188 |
| NEM666 | Nontypeable | ? | S. gordonii (95.9) | Z99190 |
| NEM1222 | Nontypeable | S. mitis (95.6) | S. mitis (97.8) | Z99192 |
| NEM1126 | Lancefield group C | S. mitis (59.5) | S. mitis (97.8) | Z99191 |
| S. mutans | ||||
| GS-5 | ND | S. mutans (99.8) | D01037 | |
| NEM1163 | Nontypeable | S. mutans (99.9) | S. mutans (99.5) | Z99193 |
| S. oralis | ||||
| CIP 103216 | ATCC 10557 | S. oralis (93.7) | Z99194 | |
| NEM1121 | Lancefield group C | S. salivarius (99.8) | S. oralis (96.1) | Z99195 |
| NEM895 | Nontypeable | ? | S. parasanguis (97.8) | Z99196 |
| S. pneumoniae | ||||
| NEM667 | Serotype 11 | ND | S. pneumoniae (100) | Z99246 |
| NEM1278 | Serotype 23F | ND | S. pneumoniae (99.8) | Z99204 |
| NEM1279 | Serotype 23F, Penr | ND | S. pneumoniae (100) | Z99205 |
| NEM1280 | Serotype 23F, Penr | ND | S. pneumoniae (100) | Z99206 |
| NEM1251 | Serotype 16 | ND | S. pneumoniae (100) | Z99201 |
| NEM1252 | Serotype 18 | ND | S. pneumoniae (100) | Z99202 |
| NEM1253 | Serotype 6 | ND | S. pneumoniae (100) | Z99203 |
| NEM1122 | Serotype 23F, Optr | ? | S. pneumoniae (99.8) | Z99200 |
| S. pyogenes | ||||
| BM105 | Lancefield group A | ND | S. pyogenes (100) | Z49247 |
| HSC5 | Lancefield group A | ND | S. pyogenes (100) | U43776 |
| S. salivarius | ||||
| CIP 102505 | ATCC 13419 | S. salivarius (?) | S. salivarius (96.3) | Z99197 |
| NEM1250 | Lancefield group D | S. salivarius (99.9) | S. salivarius (98.6) | Z99198 |
| NEM1257 | Nontypeable | S. salivarius (99.5) | S. salivarius (96.3) | Z99199 |
Data for all strains except S. agalactiae CIP 82.45 (24), S. agalactiae NEM318 (11), MG19 (5), S. gordonii BM120 (20), S. mutans GS-5 (21), S. pneumoniae NEM667 (24), S. pyogenes BM105 (24), and S. pyogenes HSC5 (13) are from this work.
Optor and Penr, resistance to optochin and penicillin, respectively.
The rapid ID 32 Strep system (API-bio-Mérieux) was used according to the manufacturer’s instructions to identify isolates to the species level. The API profiles were interpreted from the computer database for identification. The numbers in parentheses indicate the API identification percentage. ND, not determined; ?, undetermined.
The species identification was based on the phylogenic position of the sodAint fragment of the strain studied relative to those of the type strains, as indicated in Fig. 1. The numbers in parentheses indicate the percent identity of the sodAint fragment with that of the corresponding type strain.