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. 1998 Jan;36(1):77–80. doi: 10.1128/jcm.36.1.77-80.1998

FIG. 2.

FIG. 2

Sensitivity of the nested PCR with serial 10-fold dilutions of total DNA (from 500 ng to 0.5 fg) extracted from the Nine Mile VR 615 strain of C. burnetii. (A and B) Gel electrophoresis of first-round and second-round (B) PCR products amplified with primers OMP1-OMP2 and OMP3-OMP4. Lane 1, molecular size markers (100-bp DNA ladder); lanes 2 to 11, PCR products with serial 10-fold dilutions of total C. burnetii DNA (from 500 ng to 0.5 fg). (C and D) Gel electrophoresis of first-round (C) and second-round (D) PCR products amplified by primers Q3-Q5 and Q4-Q6. Lane 1, molecular size markers (100-bp DNA ladder); the DNA samples in lanes 2 to 11 are the same as those in panel A. The numbers on the sides are in base pairs.