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. Author manuscript; available in PMC: 2026 Mar 3.
Published in final edited form as: Mol Biol Cell. 2025 Jul 30;36(10):ar129. doi: 10.1091/mbc.E25-01-0033

Figure 2. The acyltransferase Sct1 antagonizes the effect of Ole1 on MDC biogenesis.

Figure 2.

(A, B) Quantification of the percentage of yeast cells overexpressing empty vector (EV), OLE1 (OLE1OE), SCT1 (SCT1OE), or both (OLE1OESCT1OE) exhibiting MDCs. Cells were treated with DMSO, concA (A) or rap (B) for 2 hours. n=3, 100 cells per n. Error bars = SEM and p-value as indicated by One Way ANOVA. (C) Super-resolution confocal fluorescence microscopy images of yeast cells overexpressing empty vector (EV), OLE1 (OLE1OE), SCT1 (SCT1OE), or both (OLE1OESCT1OE) from a GPD promoter in Chromosome I, and endogenously tagged Tom70-GFP and Tim50-mCherry. Cells were treated with DMSO or rap for 2 hours. Representative images of max projections. White arrows indicate MDCs. Scale Bar = 5 microns. (D) Quantification of (C) showing MDC diameter with mean diameter indicated along x-axis. n=3, 30-35 MDCs per n for a total of 100 MDCs. p-value as indicated by One Way ANOVA. (E) Quantification of yeast cells overexpressing empty vector (EV) or OLE1 (OLE1OE) in wild-type or sct1Δ cells. Cells were treated with DMSO or rap for 2 hours. Quantification shows the percentage of cells with MDCs. n=3, 100 cells per n. Error bars = SEM and p-value as indicated by One Way ANOVA.