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. 2025 Sep 30;12:RP90607. doi: 10.7554/eLife.90607

Table 2. List of strains used in this study.

Strain Strain Name Description Reference or Source
P. mirabilis BB2000 idrD*+pDS0062 DS349 BB2000 idrD::Tn5 (CmR) producing GFPmut2 under the aTc-inducible promoter This study
P. mirabilis BB2000 idrD*+pDS0002 DS104 BB2000 idrD::Tn5 (CmR) producing RdnE under the aTc-inducible promoter This study
P. mirabilis BB2000 idrD*+pDS0058 DS344 BB2000 idrD::Tn5 (CmR) producing RdnED39A under the aTc-inducible promoter This study
P. mirabilis BB2000 idrD*+pDS0059 DS345 BB2000 idrD::Tn5 (CmR) producing RdnEE53A under the aTc-inducible promoter This study
P. mirabilis BB2000 idrD*+pDS0060 DS346 BB2000 idrD::Tn5 (CmR) producing RdnEK55A under the aTc-inducible promoter This study
P. mirabilis BB2000 idrD*+pDS0061 DS347 BB2000 idrD::Tn5 (CmR) producing RdnED39A E53A K55A under the aTc-inducible promoter This study
E. coli MG1655 +pBBR1-NheI DS068 MG1655 carrying empty vector This study
E. coli MG1655 +pDS0002 DS151 MG1655 producing RdnE under the aTc-inducible promoter This study
E. coli MG1655 +pDS0058 DS336 MG1655 producing RdnED39A under the aTc-inducible promoter This study
E. coli MG1655 +pDS0059 DS337 MG1655 producing RdnEE53A under the aTc-inducible promoter This study
E. coli MG1655 +pDS0060 DS338 MG1655 producing RdnEK55A under the aTc-inducible promoter This study
E. coli MG1655 +pDS0061 DS339 MG1655 producing RdnED39A E53A K55A under the aTc-inducible promoter This study
P. mirabilis BB2000 idrD*+pDS0003 DS092 BB2000 idrD::Tn5 (CmR) co-producing RdnE followed by RdnI under the aTc-inducible promoter This study
E. coli MG1655 +pDS0003 DS170 MG1655 co-producing RdnE followed by RdnI under the aTc-inducible promoter This study
P. mirabilis BB2000 +pBBR1-NheI ANS1127 BB2000 carrying empty vector Wenren et al., 2013
P. mirabilis ATCC29906 +pBBR1-NheI ANS1280 ATCC29906 carrying empty vector This study
P. mirabilis ATCC29906 +pAK043 AK0132 ATCC29906 producing RdnI with a C-terminal Strep-II tag with the fla promoter This study
P. mirabilis ATCC29906 +pLMW04-gfp AK387 ATCC29906 producing GFPmut2 with the fla promoter This study
E. coli MG1655 +pDS0048 DS248 MG1655 producing RdnED39A with a C-terminal FLAG tag under an aTc-inducible promoter This study
E. coli BL21(pLysS)DE3 +pAK023 AK024 BL21(pLysS)DE3 producing RdnI with a C-terminal Strep-II tag under the T7 promoter This study
P. mirabilis ATCC29906 +pAK044 AK0135 ATCC29906 producing the RothiaRdnI with a C-terminal Strep-II tag under the fla promoter This study
P. mirabilis ATCC29906 +pAK045 AK0138 ATCC29906 producing the PrevotellaRdnI with a C-terminal Strep-II tag under the fla promoter This study
P. mirabilis ATCC29906 +pAK046 AK0141 ATCC29906 producing the PseudomonasRdnI with a C-terminal Strep-II tag under the fla promoter This study
E. coli BL21(pLysS)DE3 +pAK058 AK261 BL21(pLysS)DE3 producing RothiaRdnI with a C-terminal Strep-II tag under the T7 promoter This study
E. coli BL21(pLysS)DE3 +pAK059 AK262 BL21(pLysS)DE3 producing PrevotellaRdnI with a C-terminal Strep-II tag under the T7 promoter This study
E. coli BL21(pLysS)DE3 +pAK060 AK263 BL21(pLysS)DE3 producing PseudomonasRdnI with a C-terminal Strep-II tag under the T7 promoter This study
P. mirabilis ATCC29906 +pAK063 AK318 ATCC29906 producing the recoded ProteusRdnI sequence with a C-terminal Strep-II tag under the fla promoter This study
P. mirabilis ATCC29906 +pAK065 AK320 ATCC29906 producing the RothiaRdnI sequence (aa195-271) inserted between aa192-266 in the recoded ProteusRdnI with a C-terminal Strep-II tag under the fla promoter This study
P. mirabilis ATCC29906 +pAK066 AK321 ATCC29906 producing the PrevotellaRdnI sequence (aa170-245) inserted between aa192-266 in the recoded ProteusRdnI with a C-terminal Strep-II tag under the fla promoter This study
P. mirabilis ATCC29906 +pAK067 AK322 ATCC29906 producing the PseudomonasRdnI sequence (aa181-255) inserted between aa192-266 in the recoded ProteusRdnI with a C-terminal Strep-II tag under the fla promoter This study
P. mirabilis ATCC29906 +pAK086 AK381 ATCC29906 producing the recoded ProteusRdnI sequence (aa192-266) inserted between aa170-245 in the PrevotellaRdnI with a C-terminal Strep-II tag under the fla promoter This study
P. mirabilis ATCC29906 +pAK087 AK382 ATCC29906 producing the recoded ProteusRdnI sequence (aa192-266) inserted between aa181-255 in the PseudomonasRdnI with a C-terminal Strep-II tag under the fla promoter This study
P. mirabilis ATCC29906 +pAK064 AK319 ATCC producing the recoded ProteusRdnI sequence with seven alanine mutations (Y197A, H221A, S235A, P244A, E246A, R254A, K258A) and a C-terminal Strep-II tag under the fla promoter This study
OneShot OmniMax 2 T1R Competent Cells E. coli strain for cloning Thermo Fisher Scientific, Waltham, MA
MFDpir Mu-free E. coli mating strain to introduce plasmids into P. mirabilis Ferrières et al., 2010