| pBBR1-NheI |
empty vector with pBBR1 origin. |
Gibbs et al., 2008
|
| pLMW04-gfp |
GFPmut2 with a constitutive fla promoter, (pBBR1 origin, Kan resistance). |
Wenren et al., 2013
|
| pDS0002 |
rdnE with the anhydrotetracycline (aTc)-inducible promoter, Ptet, (pBBR1 origin, Kan resistance) |
gDS0005 was recombined into amplified pAS1054 by SliCE |
| pDS0062 |
gfpmut2 with the aTc-inducible promoter, (pBBR1 origin, Kan resistance) |
restriction digest using amplified gfpmut2 and pDS0002 |
| pDS0048 |
rdnED39A-FLAG with the aTc-inducible promoter, (pBBR1 origin, Kan resistance) |
gDS0025 was recombined into pDS0002 using restriction digest |
| pDS0058 |
rdnED39A with the aTc-inducible promoter, (pBBR1 origin, Kan resistance) |
pDS0048 was recombined into pDS0002 using restriction digest |
| pDS0059 |
rdnEE53A with the aTc-inducible promoter, (pBBR1 origin, Kan resistance) |
gDS0026 was recombined into pDS0002 using restriction digest |
| pDS0060 |
rdnEK55A with the aTc-inducible promoter, (pBBR1 origin, Kan resistance) |
gDS0027 was recombined into pDS0002 using restriction digest |
| pDS0061 |
rdnED39A, E53A, K55A with the aTc-inducible promoter, (pBBR1 origin, Kan resistance) |
gDS0028 was recombined into pDS0002 using restriction digest |
| pDS0034 |
rdnE-FLAG with the aTc-inducible promoter, (pBBR1 origin, Kan resistance) |
gDS0023 was recombined into amplified pDS0002 using SliCE |
| pDS0003 |
rdnE-rdnI with the aTc-inducible promoter, (pBBR1 origin, Kan resistance) |
Amplified rdnE-rdnI from pAS1059 was recombined into pDS0002 using SOE PCR |
| pAK023 |
rdnI-StrepII with the T7 promoter, (pUC, Amp resistance) |
gAK001 and pDS0003 were recombined into pET17b vector using SOE PCR |
| pAK043 |
rdnI-StrepII with the fla promoter, (pBBR1 origin, Kan resistance) |
rdnI-StrepII amplified from pAK023 was recombined into pLMW04 using restriction digest |
| pAK070 |
T25-linker-rdnED39A-FLAG with the IPTG-inducible lac promoter, (p15A, Kan resistance) |
rdnED39A-FLAG tag amplified from pDS0048 was recombined into pKT25 using restriction digest |
| pAK071 |
T25-linker-rdnI with the lac promoter, (p15A, Kan resistance) |
rdnI-Strep-II amplified from pAK043 was recombined into pKT25 using restriction digest |
| pAK074 |
T18-linker-rdnED39A-FLAG with the lac promoter, (Col E1 origin, Amp resistance) |
rdnED39A-FLAG tag amplified from pDS0048 was recombined into pUT18C using restriction digest |
| pAK075 |
T18-linker-rdnI-StrepII with the lac promoter, (Col E1 origin, Amp resistance) |
rdnI-Strep-II tag amplified from pAK043 was recombined into pUT18C using restriction digest |
| pAK076 |
T25-gfpmut2 with the lac promoter, (p15A origin, Kan resistance) |
Amplified gfpmut2 was recombined into pKT25 using restriction digest |
| pAK077 |
T18-gfpmut2 with the lac promoter, (Col E1 origin, Amp resistance) |
Amplified gfpmut2 was recombined into pUT18C using restriction digest |
| pAK044 |
RothiardnI-StrepII with the fla promoter, (pBBR1 origin, Kan resistance) |
gAK003 was recombined into pAK043 using restriction digest |
| pAK045 |
PrevotellardnI-StrepII with the fla promoter, (pBBR1 origin, Kan resistance) |
gAK004 was recombined into pAK043 using restriction digest |
| pAK046 |
PseduomonasrdnI-StrepII with the fla promoter, (pBBR1 origin, Kan resistance) |
gAK005 was recombined into pAK043 using restriction digest |
| pAK079 |
T18-RothiardnI-StrepII with the lac promoter, (pUC, Amp resistance) |
gAK003 was recombined into pUT18C using restriction digest |
| pAK081 |
T18-PrevotellardnI-StrepII with the lac promoter, (pUC, Amp resistance) |
gAK004 was recombined into pUT18C using restriction digest |
| pAK083 |
T18-PseudomonasrdnI-StrepII with the lac promoter, (pUC, Amp resistance) |
gAK005 was recombined into pUT18C using restriction digest |
| pAK058 |
RothiardnI-StrepII with the T7 promoter, (pUC, Amp resistance) |
gAK003 was recombined into pAK023 using restriction digest |
| pAK059 |
PrevotellardnI-StrepII with the T7 promoter, (pUC, Amp resistance) |
gAK004 was recombined into pAK023 using restriction digest |
| pAK060 |
PseudomonasrdnI-StrepII with the T7 promoter, (pUC, Amp resistance) |
gAK005 was recombined into pAK023 using restriction digest |
| pAK063 |
Sequence optimized rdnI-StrepII with the fla promoter, (pBBR1 origin, Kan resistance) |
gAK024 was recombined into pAK043 using restriction digest |
| pAK065 |
Pm/RdrdnI-StrepII (Proteus rdnI with Rothia conserved motif insert) with the fla promoter, (pBBR1 origin, Kan resistance) |
gAK026 was recombined into pAK043 using restriction digest |
| pAK066 |
Pm/PjrdnI-StrepII (Proteus rdnI with Prevotella conserved motif insert) with the fla promoter, (pBBR1 origin, Kan resistance) |
gAK028 was recombined into pAK043 using restriction digest |
| pAK067 |
Pm/PordnI-StrepII (Proteus rdnI with Pseudomonas conserved motif insert) with the fla promoter, (pBBR1 origin, Kan resistance) |
gAK027 was recombined into pAK043 using restriction digest |
| pAK086 |
Pj/PmrdnI-StrepII (Prevotella rdnI with Proteus conserved motif insert) with the fla promoter, (pBBR1 origin, Kan resistance) |
gAK029 was recombined into pAK043 using restriction digest |
| pAK087 |
Pf/PmrdnI-StrepII (Pseudomonas rdnI with Proteus conserved motif insert) with the fla promoter, (pBBR1 origin, Kan resistance) |
gAK030 was recombined into pAK043 using restriction digest |
| pAK092 |
T18-linker-Sequence optimized ProteusrdnI with a C-terminal StrepII with the lac promoter (Col E1 origin, Amp resistance) |
gAK024 was recombined into pAK075 using restriction digest |
| pAK093 |
T18-linker-Sequence optimized Pm/RardnI (Proteus rdnI with Rothia conserved motif insert) with a C-terminal StrepII with the lac promoter (Col E1 origin, Amp resistance) |
gAK026 was recombined into pAK075 using restriction digest |
| pAK094 |
T18-linker-Sequence optimized Pm/PjrdnI (Proteus rdnI with Prevotella conserved motif insert) with the lac promoter (Col E1 origin, Amp resistance) |
gAK028 was recombined into pAK075 using restriction digest |
| pAK095 |
T18-linker-Sequence optimized Pm/PfrdnI (Proteus rdnI with Pseudomonas conserved motif insert) with the lac promoter (Col E1 origin, Amp resistance) |
gAK027 was recombined into pAK075 using restriction digest |
| pAK096 |
T18-linker- Pj/PmrdnI (Prevotella rdnI with Proteus conserved motif insert) with the lac promoter (Col E1 origin, Amp resistance) |
gAK029 was recombined into pAK075 using restriction digest |
| pAK097 |
T18-linker-Pf/PmrdnI (Pseudomonas rdnI with Proteus conserved motif insert) with a C-terminal StrepII with the lac promoter (Col E1 origin, Amp resistance) |
gAK030 was recombined into pAK075 using restriction digest |
| pAK064 |
Sequence optimized rdnI7mut -StrepII with the fla promoter, (pBBR1 origin, Kan resistance) |
gAK025 was recombined into pAK043 using restriction digest |
| pAK085 |
T18-linker-Sequence optimized rdnI7mut-StrepII with the lac promoter (Col E1 origin, Amp resistance) |
gAK025 was recombined into pAK075 using restriction digest |
