Table 1.

The set of primers used for the Mycobacterium tuberculosis complex (MTBC) and nontuberculous mycobacteria (NTM) detection LAMP assay in this study.

Species (target)	Primers	Sequence (5’ to 3’)
MTBC (IS6110)	F3	GGTCGGAAGCTCCTATGA
	B3	GTTGAGCGTAGTAGGCAG
	FIP	GGGCTTGCCGGGTTTGATCAATGCACTAGCCGAGAC
	BIP	GTCCATCGAGGATGTCGAGTTGCCGCAGTACTGGTAGAGG
	LF	CAGCTCGGTCTTGTATAGGC
	LB	TGGGTCGACTGGTTCAAC
NTM (16SrRNA)	F3	CCGCGGTAATACGTAGGGT
	B3	ACCGGTGTTCCTCCTGAT
	FIP	CGCTCACAGTTAAGCCGTGAGATCCGGAATTACTGGGCGT
	BIP	GGCGATACGGGCAGACTAGAGTCGCATTCCACCGCTACAC
	LF	ACAAACCACCTACGAGCTCTT
	LB	ACTGCAGGGGAGACTGGA
M. intracellulare (MINTM006_47990)	F3	TCTTCCGTGGTACGAGAG
	B3	TGATACGGAGAAGGGATCG
	FIP	TGCACATCCTGCGCCAATCCATACATGGCGACCTTG
	BIP	GTAATCCGGCGTGGCTGTACTCCCACCAATAAGGCA
	LF	ATGGCGATCGCGATTAGG
	LB	CCATTCTGCTGGCCATCTC
M. avium (gyrB)	F3	GCCTGACCATCAACCTCACC
	B3	TGGTGGATGGGGTTTTTGG
	FIP	CTTCTCCTGCGCCGACTTGGCGGGTGACCAACGAAGAG
	BIP	AATCGGCTGCGCCGCATAAGTTTGACGAAGTCGACCAGG
	LF	TGTCGCTGACCACCTCG
	LB	TCAAGCACCGCACCTTC
M. abscessus (erm41)	F3	TGCCAGGGTGCTAGCCGTCGA
	B3	TGGCGAACAGGTGCTCGCT
	FIP	GAAATGGCCGTCGCGGTTCGTTCACGGTTTGCCGAG
	BIP	TGGTGGCGAGCCCACCAGGTCGGCAGCCA
	LF	GCGAGCAGGTCCGCTTCCGCTAT
	LB	ACCAAGTCACCAGCGCA
M. kansasii(hypothetical protein B1T47_13295)	F3	CCCGGTCCACTATTACGACA
	B3	CAGTGTCAGTGCAAGATCGA
	FIP	GGAACCGTTGATCGGTTGCCGTTCAGAATCGACCGCATGG
	BIP	CGCCAACATGGCAATCCGGACGGTCTTCCAGATCCAGCT
	LF	TGCGATGCAGGTTGCGG
	LB	GGCAGGCGGTACGTCAT
M. gordonae (ITS)	F3	CCCTTAGACACTTACAAACACT
	B3	TAAGGAGCACCACGAAGA
	FIP	CCTCCATCTTGGTGGTGGGCGAACGACAACGCATACA
	BIP	GTGTTGCCTCAGGACCCAATTCTGTAGTGGACGAAGACC
	LF	AGTGGTTGCGAGCATCAA
	LB	GCTTTGCCTGTTGTCGTG
M. fortuitum (dnaA)	F3	GATCGTGATTCCAACAGTGA
	B3	AGTCCTCGGATTCCTCTG
	FIP	GAGAGCAGAGCAAACCCCTCCACTGACTCCTCAGCAAAG
	BIP	GTTCCCACCCCGTTCGTCCTGAGCGCGTTGATGATC
	LF	TTCACCAGCTTGAGCCAG
	LB	GAGATCGAACGGCACCTC